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MicroRNAs (miRNAs) play important roles in post-transcriptional repression in nearly every biological process including germ cell development. Previously, we have identified a zebrafish germ plasm-specific miRNA  miR-202-5p , which regulates PGC migration through targeting  cdc42se1  to protect  cdc42  expression. However, knockdown of  cdc42se1  could not significantly rescue PGC migration in maternal  miR-202  mutant (M miR-202 ) embryos, indicating that there are other target genes of  miR-202-5p  required for the regulation of PGC migration. Herein, we revealed the transcriptional profiles of wild type and M miR-202  PGCs and obtained 129 differentially expressed genes (DEGs), of which 42 DEGs were enriched cell migration-related signaling pathways. From these DEGs, we identified two novel  miR-202-5p  target genes  prdm12b  and  rab10 . Furthermore, we found that disruption of  rab10  expression led to significantly migratory defects of PGC by overexpression of  rab10  siRNA, or WT, inactive as well as active forms of  rab10  mRNA, and WT  rab10  overexpression mediated migratory defects could be partially but significantly rescued by overexpression of  miR-202-5p , demonstrating that  rab10  is an important factor involved  miR-202-5p  mediated regulation of PGC migration. Taken together, the present results provide significant information for understanding the molecular mechanism by which  miR-202-5p  regulates PGC migration in zebrafish.

Proper Balance of Small GTPase rab10 Is Critical for PGC Migration in Zebrafish