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Bud endodormancy is an important, complex process subject to both genetic and epigenetic control, the mechanism of which is still unclear. The endogenous hormone abscisic acid (ABA) and its signaling pathway play important roles in the endodormancy process, in which the type 2C protein phosphatases (PP2Cs) is key to the ABA signal pathway. Due to its excellent effect on endodormancy release, hydrogen cyanamide (HC) treatment is considered an effective measure to study the mechanism of endodormancy release. In this study, RNA-Seq analysis was conducted on endodormant floral buds of pear ( Pyrus pyrifolia ) with HC treatment, and the HC-induced PP2C gene  PpPP2C1  was identified. Next, software prediction, expression tests and transient assays revealed that lncRNA  PpL-T31511 -derived  Pp-miRn182  targets  PpPP2C1 . The expression analysis showed that HC treatment upregulated the expression of  PpPP2C1  and downregulated the expression of  PpL-T31511  and  Pp-miRn182 . Moreover, HC treatment inhibited the accumulation of ABA signaling pathway-related genes and hydrogen peroxide (H 2 O 2 ). Furthermore, overexpression of  Pp-miRn182  reduced the inhibitory effect of  PpPP2C1  on the H 2 O 2  content. In summary, our study suggests that downregulation of  PpL-T31511 -derived  Pp-miRn182  promotes HC-induced endodormancy release in pear plants through the PP2C-H 2 O 2  pathway.

international journal of molecular sciences

Downregulation of lncRNA PpL-T31511 and Pp-miRn182 Promotes Hydrogen Cyanamide-Induced Endodormancy Release through the PP2C-H2O2 Pathway in Pear (Pyrus pyrifolia)