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Strain-related differences in arteriogenesis in inbred mouse strains have already been studied excessively. However, these analyses missed evaluating the mouse strain-related differences in ischemia-induced angiogenic capacities. With the present study, we wanted to shed light on the different angiogenic potentials and the associated leukocyte infiltration of  C57BL/6J  and  SV-129  mice to facilitate the comparison of angiogenesis-related analyses between these strains. For the induction of angiogenesis, we ligated the femoral artery in 8–12-week-old male  C57BL/6J  and  SV-129  mice and performed (immuno-) histological analyses on the ischemic gastrocnemius muscles collected 24 h or 7 days after ligation. As evidenced by hematoxylin and eosin staining,  C57BL/6J  mice showed reduced tissue damage but displayed an increased capillary-to-muscle fiber ratio and an elevated number of proliferating capillaries (CD31 + /BrdU +  cells) compared to  SV-129  mice, thus showing improved angiogenesis. Regarding the associated leukocyte infiltration, we found increased numbers of neutrophils (MPO +  cells), NETs (MPO + /CitH3 + /DAPI + ), and macrophages (CD68 +  cells) in  SV-129  mice, whereas macrophage polarization (MRC1 -  vs. MRC1 + ) and total leukocyte infiltration (CD45 +  cells) did not differ between the mouse strains. In summary, we show increased ischemia-induced angiogenic capacities in  C57BL/6J  mice compared to  SV-129  mice, with the latter showing aggravated tissue damage, inflammation, and impaired angiogenesis.

international journal of molecular sciences

Impact of C57BL/6J and SV-129 Mouse Strain Differences on Ischemia-Induced Postnatal Angiogenesis and the Associated Leukocyte Infiltration in a Murine Hindlimb Model of Ischemia