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The intrinsic defense mechanisms of plants toward pathogenic bacteria have been widely investigated for years and are still at the center of interest in plant biosciences research. This study investigated the role of the  AtbZIP62  gene encoding a transcription factor (TF) in the basal defense and systemic acquired resistance in Arabidopsis using the reverse genetics approach. To achieve that, the  atbzip62  mutant line (lacking the  AtbZIP62  gene) was challenged with  Pseudomonas syringae  pv.  tomato  ( Pst DC3000 ) inoculated by infiltration into Arabidopsis leaves at the rosette stage. The results indicated that  atbzip62  plants showed an enhanced resistance phenotype toward  Pst DC3000 vir  over time compared to Col-0 and the susceptible disease controls,  atgsnor1-3  and  atsid2.  In addition, the transcript accumulation of pathogenesis-related genes,  AtPR1  and  AtPR2 , increased significantly in  atbzip62  over time (0–72 h post-inoculation, hpi) compared to that of  atgsnor1-3  and  atsid2  (susceptible lines), with  AtPR1  prevailing over  AtPR2 . When coupled with the recorded pathogen growth (expressed as a colony-forming unit, CFU mL −1 ), the induction of  PR  genes, associated with the salicylic acid (SA) defense signaling, in part explained the observed enhanced resistance of  atbzip62  mutant plants in response to  Pst DC3000 vir . Furthermore, when  Pst DC3000 avrB  was inoculated, the expression of  AtPR1  was upregulated in the systemic leaves of Col-0, while that of  AtPR2  remained at a basal level in Col-0. Moreover, the expression of  AtAZI  (a systemic acquired resistance -related) gene was significantly upregulated at all time points (0–24 h post-inoculation, hpi) in  atbzip62  compared to Col-0 and  atgsnor1-3  and  atsid2 . Under the same conditions,  AtG3DPH  exhibited a high transcript accumulation level 48 hpi in the  atbzip62  background. Therefore, all data put together suggest that  AtPR1  and  AtPR2  coupled with  AtAZI  and  AtG3DPH , with  AtAZI  prevailing over  AtG3DPH , would contribute to the recorded enhanced resistance phenotype of the  atbzip62  mutant line against  Pst DC3000 . Thus, the  AtbZIP62  TF is proposed as a negative regulator of basal defense and systemic acquired resistance in plants under  Pst DC3000  infection.

international journal of molecular sciences

Enhanced Resistance of atbzip62 against Pseudomonas syringae pv. tomato Suggests Negative Regulation of Plant Basal Defense and Systemic Acquired Resistance by AtbZIP62 Transcription Factor