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Plant G proteins are versatile components of transmembrane signaling transduction pathways. The deficient mutant of heterotrimeric G protein leads to defects in plant growth and development, suggesting that it regulates the GA pathway in Arabidopsis . However, the molecular mechanism of G protein regulation of the GA pathway is not understood in plants. In this study, two G protein β subunit ( AGB1 ) mutants, agb1-2 and N692967 , were dwarfed after exogenous application of GA 3 . AGB1 interacts with the DNA-binding domain MYB62, a GA pathway suppressor. Transgenic plants were obtained through overexpression of MYB62 in two backgrounds including the wild-type ( MYB62/WT &amp;nbsp; Col-0 ) and agb1 mutants ( MYB62/agb1 ) in Arabidopsis . Genetic analysis showed that under GA 3 treatment, the height of the transgenic plants MYB62/WT and MYB62/agb1 was lower than that of WT. The height of MYB62/agb1 plants was closer to MYB62/WT plants and higher than that of mutants agb1-2 and N692967 , suggesting that MYB62 is downstream of AGB1 in the GA pathway. qRT-PCR and competitive DNA binding assays indicated that MYB62 can bind MYB elements in the promoter of GA2ox7 , a GA degradation gene, to activate GA2ox7 transcription. AGB1 affected binding of MYB62 on the promoter of GA2ox7 , thereby negatively regulating th eactivity of MYB62.

Arabidopsis G-Protein β Subunit AGB1 Negatively Regulates DNA Binding of MYB62, a Suppressor in the Gibberellin Pathway