English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Sphingosine-1-phosphate (S1P), is a signaling sphingolipid which acts as a bioactive lipid mediator. We assessed whether S1P had multiplex effects in regulating the large-conductance Ca 2+ -activated K + channel (BK Ca ) in catecholamine-secreting chromaffin cells. Using multiple patch-clamp modes, Ca 2+ imaging, and computational modeling, we evaluated the effects of S1P on the Ca 2+ -activated K + currents ( I  K(Ca) ) in bovine adrenal chromaffin cells and in a pheochromocytoma cell line (PC12). In outside-out patches, the open probability of BK Ca channel was reduced with a mean-closed time increment, but without a conductance change in response to a low-concentration S1P (1 µM). The intracellular Ca 2+ concentration (Ca i ) was elevated in response to a high-dose (10 µM) but not low-dose of S1P. The single-channel activity of BK Ca was also enhanced by S1P (10 µM) in the cell-attached recording of chromaffin cells. In the whole-cell voltage-clamp, a low-dose S1P (1 µM) suppressed I  K(Ca) , whereas a high-dose S1P (10 µM) produced a biphasic response in the amplitude of I  K(Ca) , i.e., an initial decrease followed by a sustained increase. The S1P-induced I  K(Ca) enhancement was abolished by BAPTA. Current-clamp studies showed that S1P (1 µM) increased the action potential (AP) firing. Simulation data revealed that the decreased BK Ca conductance leads to increased AP firings in a modeling chromaffin cell. Over a similar dosage range, S1P (1 µM) inhibited I  K(Ca) and the permissive role of S1P on the BK Ca activity was also effectively observed in the PC12 cell system. The S1P-mediated I  K(Ca) stimulation may result from the elevated Ca i , whereas the inhibition of BK Ca activity by S1P appears to be direct. By the differentiated tailoring BK Ca channel function, S1P can modulate stimulus-secretion coupling in chromaffin cells.

Permissive Modulation of Sphingosine-1-Phosphate-Enhanced Intracellular Calcium on BKCa Channel of Chromaffin Cells