English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Acetylcholine-induced vasorelaxation (AChIR) and responses to reduced pO 2 (hypoxia-induced relaxation (HIR), 0% O 2 ) were assessed in vitro in aortic rings of healthy male Sprague-Dawley rats (N = 252) under hyperbaric (HBO 2 ) protocols. The studied groups consisted of the CTRL group (untreated); the A-HBO 2 group (single HBO 2 ; 120 min of 100% O 2 at 2.0 bars); the 24H-HBO 2 group (examined 24 h after single exposure) and the 4D-HBO 2 group (four consecutive days of single HBO 2 ). AChIR, sensitivity to ACh and iNOS expression were decreased in the A-HBO 2 group. HIR was prostanoid- and epoxyeicosatrienoic acid (EET)-mediated. HIF-1α expression was increased in the 24H-HBO 2 and 4D-HBO 2 groups. LW6 (HIF-1α inhibitor) decreased HIR in the 24H-HBO 2 group. HBO 2 affected the expression of COX-1 and COX-2. CYP2c11 expression was elevated in the 24H-HBO 2 and 4D-HBO 2 groups. Concentrations of arachidonic acid (AA) metabolites 14(15)-DiHET, 11(12)-DiHET and 8(9)-DiHET were increased in A-HBO 2 and 24H-HBO 2. An increased concentration of 8(9)-EET was observed in the A-HBO 2 and 24h-HBO 2 groups vs. the CTRL and 4D-HBO 2 groups, and an increased concentration of 5(6)-DiHET was observed in the 24H-HBO 2 group vs. the 4D-HBO 2 group. The 20-HETE concentration was increased in the A-HBO 2 group. All were determined by LC-MS/MS of the aorta. The results show that AChIR in all groups is mostly NO-dependent. HIR is undoubtedly mediated by the CYP450 enzymes' metabolites of AA, whereas HIF-1α contributes to restored HIR. Vasoconstrictor metabolites of CYP450 enzymes contribute to attenuated AChIR and HIR in A-HBO 2 .

international journal of molecular sciences

Arachidonic Acid Metabolites of CYP450 Enzymes and HIF-1α Modulate Endothelium-Dependent Vasorelaxation in Sprague-Dawley Rats under Acute and Intermittent Hyperbaric Oxygenation