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In pancreatic islets, the major cell-types are α, β and δ cells. The γ-aminobutyric acid (GABA) signalling system is expressed in human pancreatic islets. In single hormone transcript-expressing cells, we have previously characterized the functional properties of islet GABA A receptors (iGABA A Rs). Here, we extended these studies to islet cells expressing mRNAs for more than one hormone and sought for correlation between iGABA A R activity level and relative mRNA expression ratio. The single-cell RT-PCR in combination with the patch-clamp current recordings was used to examine functional properties of iGABA A Rs in the multiple hormone mRNA-expressing cells. We detected cells expressing double (α/β, α/δ, β/δ cell-types) and triple (α/β/δ cell-type) hormone transcripts. The most common mixed-identity cell-type was the α/β group where the cells could be grouped into β- and α-like subgroups. The β-like cells had low GCG / INS expression ratio (&lt;0.6) and significantly higher frequency of iGABA A R single-channel openings than the α-like cells where the GCG / INS expression ratio was high (&gt;1.2). The hormone expression levels and iGABA A R single-channel characteristics varied in the α/β/δ cell-type. Clearly, multiple hormone transcripts can be expressed in islet cells whereas iGABA A R single-channel functional properties appear to be α or β cell specific.

GABAA Receptor-Mediated Currents and Hormone mRNAs in Cells Expressing More Than One Hormone Transcript in Intact Human Pancreatic Islets