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One of the main applications of field spectroscopy is the generation of spectral libraries of Earth’s surfaces or materials to support mapping activities using imaging spectroscopy. To enhance the reliability of these libraries, spectral signature acquisition should be carried out following standard procedures and controlled experimental approaches. This paper presents a standard protocol for the creation of a spectral library for plant species. The protocol is based on characterizing the reflectance spectral response of different species in the spatiotemporal domain, by accounting for intra-species variation and inter-species similarity. A practical case study was conducted on the shrubland located in Doñana National Park (SW Spain). Spectral libraries of the five dominant shrub species were built (Erica scoparia, Halimium halimifolium, Ulex australis, Rosmarinus officinalis, and Stauracanthus genistoides). An estimation was made of the separability between species: on one hand, the Student’s t-test evaluates significant intra-species variability (p < 0.05) and on the other hand, spectral similarity value (SSV) and spectral angle mapper (SAM) algorithms obtain significant separability values for dominant species, although it was not possible to discriminate the legume species Ulex australis and Stauracanthus genistoidesWe acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI).The authors are extremely grateful to the staff of the INTA Remote Sensing Laboratory for their\udscientific support and the staff of the Doñana Biological Research Station for their outstanding logistical\udsupport received in the course of the workPeer reviewe

Towards a Standard Plant Species Spectral Library Protocol for Vegetation Mapping: A Case Study in the Shrubland of Doñana National Park