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Superparamagnetic polymers (SPMP) microbead was an excellent form fornanosized magnetic particles less than 10nm to realize their potential applications in manyfields. We developed a novel modified suspension polymerization method for the productionof superparamagnetic poly (methacrylate divinylbenzene) (PMA-DVB) microbeads, withamino groups on their surface after simple modifications. We applied these SPMPmicrobeads to establish a sandwich enzyme chemiluminence immuno (ECLIA) procedure ofdetecting the free hCGÃŽÂ² in serum. It was proved to a better method compared to the ELISA,since it need half of the sample volume, simpler protocols, and the time it need wasshortened from 2 hours to 1 hour. The detection limit was 0.22 mIUÃ¢Â€Â¢m-1, one order lowerthan the ELISA assay. Its linear range was between 0.45Ã¢Â€Â”185.2 mIUÃ¢Â€Â¢m-1. And in thedetections of clinical serum samples, the related coefficient between the two methodsÃ¢Â€Â™results was 0.955

Enzyme Chemiluminescence Immuno Assay of Free hCGβ in Serum Based on Superparamagnetic Polymer Microbeads