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GLOBOSA  ( GLO ), a B-class MADS-box gene, is involved in floral organ determination but has rarely been studied in  Osmanthus fragrans , which is a very popular ornamental tree species in China. Here, the full-length cDNA of a homologous  GLO1  gene (named  OfGLO1 ) was cloned from a flower bud of  O. fragrans  using the RACE technique. The  OfGLO1  has a 645 bp open reading frame, encoding 214 amino acids. Similar to other PI/GLO proteins, OfGLO1 has two conserved domains, MADS MEF2-like and K-box, and a 16-amino-acid PI motif in the C terminal region. Our phylogeny analysis classified OfGLO1 as a PI-type member of the B-class MADS-box gene family. The qRT-PCR assay showed that the expression of  OfGLO1  in  O. fragrans  was continuously upregulated from the tight bud stage to the full flowering stage but barely expressed in the pistils, sepals, and non-floral organs, such as root, leaf, and stem. The genetic effect of  OfGLO1  was assayed by ectopic expression in tobacco plants. Compared with the wild-type,  OfGLO1  transformants showed reduced plant size, earlier flowering, shorter stamens, and lower seed setting rates. Furthermore, some stamens were changed into petal-like structures. These findings indicate that  OfGLO1  plays an important role in the regulation of flower development. This study improved our understanding of class B gene function in woody plants.

Cloning, Expression, and Tobacco Overexpression Analyses of a PISTILLATA/GLOBOSA-like (OfGLO1) Gene from Osmanthus fragrans