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This study analysed the effect of prior high-pressure processing (HPP; 200-600 MPa, 2 min), freezing (-30 °C, 48 h), and frozen storage (-18 °C, 6 months) on the macroelement and trace element content in brine-canned mackerel ( Scomber colias ). Most elements ( Na , Ca , Ba , Mn , Fe , Cu , Cd , Sn , As , S, and Se ) showed an increased ( p &lt; 0.05) presence in mackerel muscle canned after freezing. A content increase ( p &lt; 0.05) was also observed for Na and Sn if prior frozen storage was also applied; on the contrary, Ca , Ba , Mn , Fe , Cd , S , and Se showed a content decrease ( p &lt; 0.05) as a result of such storage. Freezing, frozen storage, and canning led to lower values ( p &lt; 0.05) in canned fish for K , Mg , Pb , and P . Prior HPP led to relevant content decreases ( p &lt; 0.05) for K , Mg , Ca , Ba , Mn , Fe , Pb , and P contents in fish canned after the freezing step; HPP provoked additional decreases ( p &lt; 0.05) in Ca , Ba , and Mn levels in samples corresponding to 6-month frozen storage. On the contrary, prior HPP led to marked increases ( p &lt; 0.05) for Cd , S , and Se contents in all canned samples. Content changes are explained on the basis of modifications of other constituents and liquor losses from muscle.

Macroelements and Trace Elements Content in Brine-Canned Mackerel (Scomber colias) Subjected to High-Pressure Processing and Frozen Storage