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Abies spp. in general have been shown to need a period of cold stratification  to break dormancy and germinate, but this can be very time consuming. In this study, hydropriming by itself and in combination with biopriming was carried out on Abies hickelii and Abies religiosa seeds. For biopriming, three species of plant growth promoting rhizobacteria ( Pseudomonas fluorescens, P. putida and Bacillus subtilis) were tested. The purpose was to determine if germination and growth could be improved for these two endangered species. Our results demonstrated that treating A. hickelii and A. religiosa with both hydropriming and biopriming with certain strains of Plant Growth-Promoting Rhizobacteria (PGPR) could improve germination rates up to 91% for A. hickelii and up to 68% for A. religiosa. Importantly, these treatments showed no significant negative impact on the growth of A. religiosa and actually improved growth in A. hickelii. The application of both hydropriming and biopriming offer possibly an alternative methodology to improve germination, survival and preservation of these fir tree species of Mexico that are at risk  of extinction

Effect of Hydropriming and Biopriming on Seed Germination and Growth of Two Mexican Fir Tree Species in Danger of Extinction