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The flow cytometer is an instrument that can measure the characteristics of cells such as the number of cells, the degree of internal composition of the cells, the size of the cells, and the cell cycle etc. This equipment has been used to study leukemia, DNA and RNA analysis, protein expression, cell death, and immune response. However, a flow cytometer is expensive equipment and requires an operator with expertise for use and maintenance. When only simple data are needed, such as measuring the number of cells or quantitative analysis of cell growth and inhibition, the use of a flow cytometer is not suitable in terms of cost and requires unnecessary measurement time consumption. In this study, a compact optical measurement system using commercially available light-emitting diodes (LED), photodiode, and Arduino Mega ADK was developed, and the body structure was printed and utilized by a 3D printer. Cervical cancer cells, known as one of the major cancers of women, were fluorescently treated with fluorescent dyes such as Calcein-AM and DiD, and performance of the system was verified. The side scattering measured using various filters with different transmission wavelengths of light showed high linearity in proportion to the number of cells. By measuring the side scattering of the untreated cervical cancer cells, fluorescence scattering could be confirmed from the difference in the side scattering intensity according to the fluorescence treatment.

Development of a Compact Optical Measurement System to Quantify the Optical Properties of Fluorescently Labeled Cervical Cancer Cells