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The increasing incidence of methicillin-resistant and biofilm-forming  S. aureus  isolates in hospital settings is a gruesome concern today. The main objectives of this study were to determine the burden of  S. aureus  in clinical samples, assess their antibiotic susceptibility pattern and detect biofilm formation and  mec A gene in them. A total of 1968 different clinical specimens were processed to isolate  S. aureus  following standard microbiological procedures. Antibiotic susceptibility test of the isolates was performed by Kirby–Bauer disc-diffusion method following CLSI guidelines. Biofilm was detected through tissue culture plate method. Methicillin-resistant  S. aureus  (MRSA) isolates were screened using cefoxitin (30 µg) discs and  mec A gene was amplified by conventional polymerase chain reaction (PCR). Of 177 bacterial growth, the prevalence of  S. aureus  was 15.3% ( n  = 27). MRSA were 55.6% (15/27) and 44% (12/27) exhibited multidrug resistance (MDR). There was no significant association between methicillin resistance and MDR ( p  > 0.05). Both MRSA and MSSA were least sensitive to penicillin (100%, 75%) followed by erythromycin (86.6%, 66.6%). Most of the MRSA (93.4%) were susceptible to tetracycline. All  S. aureus  isolates were biofilm producers—19 (70%) were weak and only one (4%) was a strong biofilm producer. The strong biofilm-producing MSSA was resistant to most of the antibiotics except cefoxitin and clindamycin. None of the MSSA possessed  mec A gene while 8 (53.3%) MRSA had it. More than half of  S. aureus  isolated were MRSA. High incidence of multidrug resistance along with capacity to form biofilm among clinical isolates of  S.   aureus  is a matter of apprehension and prompt adoption of biosafety measures is suggested to curb their dissemination in the hospital environments.

Antibiotic Susceptibility, Biofilm Production, and Detection of mecA Gene among Staphylococcus aureus Isolates from Different Clinical Specimens