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Adding crowding agents such as polyethylene glycol (PEG) to lyotropic chromonic liquid crystals (LCLCs) formed by water dispersions of materials such as disodium cromoglicate (DSCG) leads to a phase separation of the isotropic phase and the ordered phase. This behavior resembles nanoscale condensation of DNAs but occurs at the microscale. The structure of condensed chromonic regions in crowded dispersions is not yet fully understood, in particular, it is not clear whether the condensed domains are in the nematic (N) or the columnar (C) state. In this study, we report on small angle X-ray scattering (SAXS) and wide-angle X-ray scattering (WAXS) measurements of mixtures of aqueous solutions of DSCG with PEG and compare results to measurements of aqueous solutions of pure DSCG. X-ray measurements demonstrate that addition of PEG to DSCG in the N phase triggers appearance of the C phase that coexists with the isotropic (I) phase. Within the coexisting region, the lateral distance between the columns of the chromonic aggregates decreases as the temperature is increased.

Effect of Crowding Agent Polyethylene Glycol on Lyotropic Chromonic Liquid Crystal Phases of Disodium Cromoglycate