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Janus particles are known to be useful to various fields such as biomolecule-probing sensors, reaction catalysts, surfactants, and so on. They have two chemically different surfaces which possess contradictory characteristics such as polarity, hydrophobicity, etc. Here, a simple fabrication of dumbbell-shaped Janus microparticles was tested by the chemical reaction of carboxyl groups and amino groups to form amide bonds. They were distributed to the interface between polyethylene glycol (PEG)-rich phase and dextran (DEX)-rich phase, while the unreacted particles having carboxyl groups located at the top PEG-rich phase and particles having amine ligands went to the bottom DEX-rich phase of an aqueous two-phase system (ATPS). The fabrication procedures, observations, and possible applications of results are discussed.

Partitional Behavior of Janus Dumbbell Microparticles in a Polyethylene Glycol (PEG)-Dextran (DEX) Aqueous Two-Phase System (ATPS)