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Microalgae oil has been regarded as a promising feedstock for biodiesel production. However, microalgae oil usually contains some non-lipid components, such as pigments. Microalgae oil could be converted to biodiesel effectively with a two-step process to decrease the negative effect caused by by-product glycerol generated in traditional biodiesel production process. Firstly, microalgae oil was hydrolysed to free fatty acids (FFAs) and then FFAs were converted to methyl ester. In this study, the hydrolysis of microalgae oil from Schizochytrium sp. was systematically investigated and microalgae oil could be hydrolysed effectively to FFAs at both non-catalytic and acid-catalytic conditions. The hydrolysis degree of 97.5% was obtained under non-catalytic conditions of 220 ◦C and a water to oil ratio of 10:1 (w:w). The hydrolysis degree of 97.1% was obtained with the optimized sulphuric acid catalytic conditions of 95 ◦C, and a ratio of water to oil 3:1. The lipase Novozym435-mediated esterification with the hydrolysed FFAs was explored and a FAME (Fatty Acids Methyl Ester) yield of 95.1% was achieved. The conversion of different FFAs also was compared and the results indicated that lipase Novozym435-mediated methanolysis was effective for the preparation of biodiesel as well as poly unsaturated fatty acids (PUFAs).

Efficient Biodiesel Conversion from Microalgae Oil of Schizochytrium sp.