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Pathogenesis-related proteins (PRs) are induced in plants after infection by pathogens and/or abiotic stress. Among these proteins, the family 10 (PR-10) influences the biosynthesis of secondary metabolites and shows antimicrobial ribonuclease activity. Tc PR-10p (Pathogenesis-related Protein 10 of Theobroma cacao ) was isolated from resistant and susceptible Moniliophthora perniciosa cacao cultivars. Cell survival with Saccharomyces cerevisiae mutant lines deficient in ATP-binding cassette (ABC) transporter proteins indicated the influence on resistance to Tc PR-10p. Proteins of the ABC transport type are considered important in the process of resistance to antimicrobials and toxins. Thus, the objective of this work was to observe the sensitivity of ABC transporter yeast mutants in the presence of the Tc PR-10p. Chronic exposure of S. cerevisiae mitochondrial (BY atm1&amp;Delta; and BY mdl1&amp;Delta; ) and vacuole (BY nft1&amp;Delta; , BY vmr1&amp;Delta; , BY ybt1&amp;Delta; , BY ycf1&amp;Delta; and BY bpt1&amp;Delta; ) ABC transporter mutants to Tc PR-10p (3 &amp;mu;g/mL, 0, 6, 12 and 24 h) was performed. Two Tc PR-10p sensitive strains (BY mdl1&amp;Delta; and BY nft1&amp;Delta; ) were submitted to a fluorescence test with the fluorogenic dihydroethidium (DHE), to visualize the presence of oxidative stress in the cells. Oxidative stress-increased sensitivity was confirmed by flow cytometry indicating induced cell death either via apoptosis or necrosis. This yeast data combined with previous data of literature (of M. perniciosa sensitivity to Tc PR-10p) show that increased sensitivity to Tc PR-10p in these mutants could be due to the Tc PR10p-generated higher levels of intracellular reactive oxygen species (ROS), leading to increased cell death either via necrosis or apoptosis.

Sensitivity of Yeast Mutants Deficient in Mitochondrial or Vacuolar ABC Transporters to Pathogenesis-Related Protein TcPR-10 of Theobroma cacao