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Optical tweezers can manipulate micro-particles, which have been widely used in various applications. Here, we experimentally demonstrate that optical tweezers can assemble the micro-particles to form stable structures at the glass–solution interface in this paper. Firstly, the particles are driven by the optical forces originated from the diffraction fringes, which of the trapping beam passing through an objective with limited aperture. The particles form stable ring structures when the trapping beam is a linearly polarized beam. The particle distributions in the transverse plane are affected by the particle size and concentration. Secondly, the particles form an incompact structure as two fan-shaped after the azimuthally polarized beam passing through a linear polarizer. Furthermore, the particles form a compact structure when a radially polarized beam is used for trapping. Thirdly, the particle patterns can be printed steady at the glass surface in the salt solution. At last, the disadvantage of diffraction traps is discussed in application of optical tweezers. The aggregation of particles at the interfaces seriously affects the flowing of particles in microfluidic channels, and a total reflector as the bottom surface of sample cell can avoid the optical tweezers induced particle patterns at the interface. The optical trapping study utilizing the diffraction gives an interesting method for binding and assembling microparticles, which is helpful to understand the principle of optical tweezers.

Optical Assembling of Micro-Particles at a Glass–Water Interface with Diffraction Patterns Caused by the Limited Aperture of Objective