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An efficient microwave-assisted simultaneous distillation and extraction (MA-SDE) method has been developed for the separation of polysaccharides and essential oil from Taxus chinensis var. mairei. The key operating parameters for MA-SDE were optimized by single factor and central composite design experiments, and the optimal conditions were found to include a particle size of 60–80 mesh, liquid/solid ratio of 22.5 mL/g, extraction time of 17.5 min, microwave power of 547 W, and dichloromethane was used as the extraction solvent of the essential oil. The yields obtained for polysaccharides and essential oil under the optimized conditions were 6.39% ± 0.12% and 0.27% ± 0.03%, respectively. The MA-SDE method was also compared with conventional heat reflux extraction (HRE) and hydrodistillation extraction (HDE). The MA-SDE method not only allowed for the simultaneous extraction of polysaccharides and essential oil, but also completed the task with a much shorter extraction time of 17.5 min (HRE and HDE required 3 and 6 h, respectively). Furthermore, the MA-SDE method gave increased extraction yields for polysaccharides (1.14-fold higher than HRE) and essential oil (1.23-fold higher than HDE). Based on these results, this MA-SDE method represents a rapid and efficient technique for the simultaneous extraction of polysaccharides and essential oil

applied sciences

A Microwave-Assisted Simultaneous Distillation and Extraction Method for the Separation of Polysaccharides and Essential Oil from the Leaves of Taxus chinensis Var. mairei