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Current and future research focuses on the use of renewable technologies and materials to stabilise weak soils, of varying degrees, for road construction applications. Soil stabilisation is a method of strengthening a natural soil to meet this purpose. Our interest is in the use of bio-based components, derived from microbial growth processes, that contribute to the needed desirable strength characteristics for in situ soil stabilisation. This investigation focuses on novel Bacillus-based stabilisers obtained from the vegetative and spore growth stage. In this study, eighteen bio-based components were derived from a Bacillus licheniformis fermentation and extracted into various aqueous and non-aqueous fractions for strength property assessment. The strength properties of the treated soils (i.e., dolerite and weathered granite soil) were assessed via previously developed lab-scale equipment to rapidly pre-select the best performing fractions, (i.e., compression stress, erosion, abrasion, and water absorption tests). The effect of one superior performing prototype (a) was validated at large-scale, using standard erosion and abrasion tests (i.e., whole broth at 1.8% stabiliser concentrations), and showed resistance to abrasion (3.37 ± 0.03%) (p value ≤ 0.0001) and resistance to erosion (33.20 ± 0.15%) (p value ≤ 0.001). The elemental composition and microstructure of the bio-stabilised soil was determined using energy dispersive X-ray spectroscopy and scanning electron microscopy, respectively. This evaluation formed part of the selection of the best performing Bacillus derived fractions and achieved a proof of concept for the next phase of product prototype development. This study demonstrated a novel bio-mediated approach to the overall criteria for evaluation and selection of candidate product prototype/s, for stabilisation of two varying soils, and for potential application in road construction works.

Performance Evaluation of Bio-Based Fractions Derived from Bacillus spp. for Potential In Situ Soil Stabilisation