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Quantitative  analysis  of  carotenoids  has  been  extensively  reported  using  UV‐Vis spectrophotometry and chromatography, instrumental techniques that require complex extraction protocols with  organic  solvents.  Fourier  transform  infrared  spectroscopy  (FTIR)  is  a  potential alternative for simplifying the analysis of food constituents. In this work, the application of FTIR with attenuated  total  reflectance  (ATR) was  evaluated  for  the determination of  total  carotenoid content  (TCC)  in Cucurbita spp. samples. Sixty‐three samples, belonging  to different cultivars of butternut  squash  (C.  moschata)  and  pumpkin  (C.  maxima),  were  selected  and  analyzed  with FTIR‐ ATR  (attenuated  total  reflectance). Three different preparation protocols  for samples were followed: homogenization (A), freeze‐drying (B), and solvent extraction (C). The recorded spectra were used  to develop  regression models by Partial Least  Squares  (PLS), using data  from TCC, determined by UV‐Vis spectrophotometry. The PLS regression model obtained with the FTIR data from the freeze‐dried samples, using the spectral range 920–3000 cm−1, had the best figures of merit (R2CAL of 0.95, R2PRED of 0.93 and RPD of 3.78), being reliable for future application in agriculture. This approach for carotenoid determination in pumpkin and squash avoids the use of organic solvents. Moreover,  these results are a rationale  for  further exploring  this technique  for  the assessment of specific carotenoids in food matrices.

FTIR-ATR Spectroscopy Combined with Multivariate Regression Modeling as a Preliminary Approach for Carotenoids Determination in Cucurbita spp.