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Developing fast and accurate fluorescence detection technology of oil spill is significant for quantitative analysis in unexpected oil spill events. As the oil sample concentration increases, the fluorescence spectrum produces red‐shift behavior, which seriously affects the quantitative detection of concentration. In this work, a three‐dimensional concentration‐emission matrix (CEM) was constructed by using a series of emission spectra with different levels of concentration at the excitation wavelength of 266 nm. The database is the interpolated CEM of six samples using bicubic interpolation in the concentration dimension. With matrix similarity matching, the database was used to achieve quantification of the concentration of oil samples. The recovery rates of prediction for test samples and weathering samples of six oil samples were between 86.8% and 115.57%, with relative errors of predictions ranging from 2.09% to 15.2%. The results show that this method can provide accurate quantitative determination of the concentration of different oil samples.

Concentration Quantification of Oil Samples by Three-Dimensional Concentration-Emission Matrix (CEM) Spectroscopy