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Carotenoids function as photosynthetic accessory pigments, antioxidants, and vitamin A precursors. We recently showed that transgenic sweetpotato calli overexpressing the mutant sweetpotato ( Ipomoea batatas [L.] Lam) Orange gene ( IbOr - R96H ), which carries a single nucleotide polymorphism responsible for Arg to His substitution at amino acid position 96, exhibited dramatically higher carotenoid content and abiotic stress tolerance than calli overexpressing the wild-type IbOr gene ( IbOr - WT ). In this study, we generated transgenic sweetpotato plants overexpressing IbOr - R96H under the control of the cauliflower mosaic virus (CaMV) 35S promoter via Agrobacterium -mediated transformation. The total carotenoid contents of IbOr - R96H storage roots (light-orange flesh) and IbOr - WT storage roots (light-yellow flesh) were 5.4-19.6 and 3.2-fold higher, respectively, than those of non-transgenic (NT) storage roots (white flesh). The β-carotene content of IbOr - R96H storage roots was up to 186.2-fold higher than that of NT storage roots. In addition, IbOr - R96H plants showed greater tolerance to heat stress (47 °C) than NT and IbOr - WT plants, possibly because of higher DPPH radical scavenging activity and ABA contents. These results indicate that IbOr - R96H is a promising strategy for developing new sweetpotato cultivars with improved carotenoid contents and heat stress tolerance.

Overexpression of the Golden SNP-Carrying Orange Gene Enhances Carotenoid Accumulation and Heat Stress Tolerance in Sweetpotato Plants