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Feathers are naturally made up of non-digestible proteins. Under thermal processing, total tract digestibility can be partially improved. Furthermore, Bacillus subtilis (Bs) has shown a hydrolytic effect In vitro. Then, a Bs FTC01 was selected to hydrolyze enough feathers to produce a meal, and then test the quality and inclusion in the dog’s diet to measure the apparent total tract digestibility coefficient (ATTDC) in vivo and the microorganism’s ability to survive in the gastrointestinal tract. A basal diet was added with 9.09% hydrolyzed Bs feather meal (HFMBs) or 9.09% thermally hydrolyzed feather meal (HFMT). Nine adult dogs were randomized into two 10-day blocks and fed different diets. Microbial counts were performed on feather meal, diets and feces. The Bs was less effective in digesting the feathers, which reduced the ATTDC of dry matter, crude protein, energy and increased the production of fecal DM, but the fecal score was maintained (p &gt; 0.05). The digestible energy of HFMT and HFMBs was 18,590 J/kg and 9196 J/kg, respectively. Bacillus subtilis showed limitation to digest feather in large scale, but the resistance of Bs to digestion was observed since it grown on feces culture.

Determination of the Nutritional Value of Diet Containing Bacillus subtilis Hydrolyzed Feather Meal in Adult Dogs