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The nettle Urtica  cannabina ( U.  cannabina ) is not only a functional edible food for humans but also a potential alternative feed resource for poultry, providing protein, fatty acids, vitamins, and minerals. The present study was conducted to evaluate the effects of dietary U.  cannabina on the production of high-quality eggs with enriched n-3 polyunsaturated fatty acids (PUFA) and lower cholesterol contents. One hundred and twenty laying hens were assigned to three groups [control, 15% alfalfa meal supplementation (TRTA), and 15% U.  cannabina supplementation (TRTU)]. The results showed that the feed intake, yolk color, and shell thickness were increased ( p &lt; 0.05) in the U.  cannabina group. Moreover, cholesterol contents of the yolk and serum were lower in the U.  cannabina group ( p &lt; 0.05). The total n-3 PUFA concentration in the yolk was increased while the ratio of n-6/n-3 was reduced in the U.  cannabina group ( p &lt; 0.01). In conclusion, the dietary inclusion of U.  cannabina increased shell thickness, yolk n-3 PUFA levels, and yolk color, and reduced cholesterol contents of the yolk and serum without any negative impacts on health or laying performance.

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Thicker Shell Eggs with Enriched N-3 Polyunsaturated Fatty Acids and Lower Yolk Cholesterol Contents, as Affected by Dietary Nettle (Urtica cannabina) Supplementation in Laying Hens