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One way to face the consequences of climate change and the expected increase in water availability in agriculture is to find genotypes that can sustain production at a lower water cost. This theoretically can be achieved by using genetic material with an increased water use efficiency. We compared the leaf Water Use Efficiency (WUEi) under realistic field conditions in 14 vine genotypes of the Tempranillo cultivar (clones), in two sites of Northern Spain for three and five years each to evaluate (1) if a clonal diversity exists for this traits among those selected clones and (2) the stability of those differences over several years. The ranking of the different clones showed significant differences in WUEi that were maintained over years in most of the cases. Different statistical analyses gave coincident information and allowed the identification of some clones systematically that had a higher WUEi or a lower WUEi. These methods also allowed the identification of the underlying physiological process that caused those differences and showed that clones with a higher WUEi are likely to have an increased photosynthetic capacity (rather than a different stomatal control). Those differences could be useful to orientate the decision for vines selection programs in the near future.

Variability in Water Use Efficiency of Grapevine Tempranillo Clones and Stability over Years at Field Conditions