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Recently, large-scale agriculture has led to increasing crop production. To increase crop productivity in large-scale cropping systems, attempts have been made to make nano-fertilizers and deliver them to the crops by extension of nanotechnology. Hence, nano-fertilizers might be defined as nanoparticles that may directly assist in supplying essential nutrients for crop productivity. Seed germination is the first and the most susceptible stage in the plant’s growing phases, so could be considered as an index to evaluate the effect of newly developed materials such as nanoparticles (NPs), providing useful information for researchers. In our experiments, germination tests have been carried out in Petri dishes containing wet filter paper and nano-primed seeds. We had biosynthesized seven nanoparticles in our previous studies including calcinated and non-calcinated zinc oxide, zinc, magnesium oxide, silver, copper, and iron nanoparticles. The effect of these biogenic nanoparticles and their counterpart metallic salts including zinc acetate, magnesium sulfate, silver nitrate, copper sulfate, and iron (III) chloride was studied on two popularly grown plants, wheat and flax, in laboratory conditions to obtain preliminary information for future field experiments. Germination percentage, shoot length, root length, seedlings length, root–shoot ratio, seedling vigor index (SVI), shoot length stress tolerance index (SLSI), and root length stress tolerance index (RLSI) were calculated on the second and seventh days of the experiment. According to the results, the response of the plants to metal containing nanoparticles and metal salts mainly depend on the type of the metal, plant species, concentration of the NP suspension or salt solution, condition of the exposure, and the stage of growth.

Ameliorating Seed Germination and Seedling Growth of Nano-Primed Wheat and Flax Seeds Using Seven Biogenic Metal-Based Nanoparticles