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Nitrogen (N) fertilizer plays a vital role in increasing cotton yield, but its excessive application leads to lower yield, lower nitrogen use efficiency (NUE), and environmental pollution. The main objective of this study was to find an effective method to enhance the NUE in cotton production. A two-year field experiment was conducted by using a split plot design with N rates (N0, 0 kg N ha−1; N1, 112.5 kg N ha−1; N2, 225 kg N ha−1, N3, 337.5 kg N ha−1), and cotton cultivars (CRI 69; ZZM 1017; ZZM GD89 and XLZ 30) to evaluate both their individual effect and their interactions on cotton yield and NUE. The results showed that the biomass and N accumulation of four cultivars increased with an increase in N rate, whereas the NUE decreased. Cotton yield increased first and then decreased for CRI 69 and ZZM 1017, while kept increasing from N0 to N3 for ZZM GD89 and XLZ 30. Compared with ZZM GD89 and XLZ 30, CRI 69, and ZZM 1017 showed higher yield, resulted from higher biomass, bolls per plant, and boll weight, especially under low N level. In addition, the CRI 69 and ZZM 1017 had stronger N absorption and transformation capabilities, and showed higher NUE than those of ZZM GD89 and XLZ 30 under the same N rate. The results indicated that CRI 69 and ZZM 1017 show advantages over ZZM GD89 and XLZ 30 in yield, and NUE, especially under low N rate.

N-Use Efficiency and Yield of Cotton (G. hirsutumn L.) Are Improved through the Combination of N-Fertilizer Reduction and N-Efficient Cultivar