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Preparation of two ligands acetonesemicarbazone (AScH) and 2,4-dioximepentane (DOXPH2) using three ways {includes reflux method, heating using microwave rays technique and ultrasonic technique}, and characterized using physical and spectral techniques. Followed by the preparation of new Ni(II) complexes containing mixed of three ligands {including (AScH), (DOXPH2) and 2-ethoxyethanol (EtO2H). The complexes have been characterized using quantitative, physical and spectral techniques. The electrical conductivity indicate that the complexes prepared in slightly neutral medium (pH=6-7) are electrolytes (1:2), meanwhile the complexes prepared in alkaline medium are nonelectrolytes. All the ligands above acts as bidentate chelating ligands., and octahedral geometries of the complexes are resulted. The effect of laser have been studied on solid ligands and complexes, no effects have been observed on all the compounds through the results of melting or decomposition point, conductivity and electronic spectra, this means that all the compounds are stable and are not affected by this kind of radiation. Also the biological activity of all the prepared compounds have been evaluated against pathogenic bacterial strains (Staphylococcus aureus, Streptococcus species and E. coli), all the compounds were found to have no antibacterial activity .

New Mixed Ligands With Nickel (II) Complexes, Study of Their Physical & Spectral Properties , Antibacterial Activities and Laser Irradiation