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Spiroplasma  are vertically-transmitted endosymbionts of ticks and other arthropods. Field-collected  Ixodes persulcatus  have been reported to harbour  Spiroplasma , but nothing is known about their persistence during laboratory colonisation of this tick species. We successfully isolated  Spiroplasma  from internal organs of 6/10 unfed adult ticks, belonging to the third generation of an  I. persulcatus  laboratory colony, into tick cell culture. We screened a further 51 adult male and female ticks from the same colony for presence of  Spiroplasma  by genus-specific PCR amplification of fragments of the 16S rRNA and  rpoB  genes; 100% of these ticks were infected and the 16S rRNA sequence showed 99.8% similarity to that of a previously-published  Spiroplasma  isolated from field-collected  I. persulcatus . Our study shows that  Spiroplasma  endosymbionts persist at high prevalence in colonised  I. persulcatus  through at least three generations, and confirms the usefulness of tick cell lines for isolation and cultivation of this bacterium.

Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks