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The ecology and host feeding patterns of many soft ticks (Ixodida: Argasidae) remain poorly understood. To address soft tick–host feeding associations, we fed  Ornithodoros turicata  Dugès on multiple host species and evaluated quantitative PCR (qPCR) and stable isotope analyses to identify the vertebrate species used for the bloodmeal. The results showed that a qPCR with host-specific probes for the  cytochrome b  gene successfully identified bloodmeals from chicken ( Gallus gallus  L.), goat ( Capra aegagrus hircus  L), and swine ( Sus scrofa domesticus ) beyond 330 days post-feeding and through multiple molting. Also, qPCR-based bloodmeal analyses could detect multiple host species within individual ticks that fed upon more than one species. The stable isotope bloodmeal analyses were based on variation in the natural abundance of carbon ( 13 C/ 12 C) and nitrogen ( 15 N/ 14 N) isotopes in ticks fed on different hosts. When compared to reference isotope signatures, this method discerned unique δ 13 C and δ 15 N signatures in the ticks fed on each host taxa yet could not discern multiple host species from  O. turicata  that fed on more than one host species. Given the significance of soft tick-borne zoonoses and animal diseases, elucidating host feeding patterns from field-collected ticks using these methods may provide insight for an ecological basis to disease management.

Identification of Host Bloodmeal Source in Ornithodoros turicata Dugès (Ixodida: Argasidae) Using DNA-Based and Stable Isotope-Based Techniques