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To develop an attenuated vaccine candidate against K88ac enterotoxigenic  Escherichia coli  (ETEC), a novel  Escherichia coli  ( E. coli ) K88ac  LT(S63K) Δ STb  with  LT(S63K)  mutation and  ST1  deletion was generated using site mutagenesis and λ-Red homologous recombination based on wild paternal ETEC strain C83902.  E. coli  K88ac  LT(S63K) Δ STb  showed very similar fimbriae expression and growth kinetics to the wild strain C83902, but it was significantly attenuated according to the results of a rabbit ligated ileal loop assay and mouse infection study. Oral inoculation with  E. coli  K88ac  LT(S63K) Δ STb  stimulated the mucosa immune response and induced the secretion of IgA to K88ac in the intestines in mice. A challenge experiment revealed that the attenuated strain provided efficient protection against C83902 in the following 7 days and at the 24th day post-inoculation, suggesting that the attenuated isolate could act as an ecological protectant and vaccine in preventing K88ac ETEC.

An Attenuated Escherichia coli K88ac LT(S63K)ΔSTb Efficiently Provides Protection Against Enterotoxigenic Escherichia coli in the Mouse Model