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Salmonella enteritidis  and  Salmonella pullorum  belonging to Group O 9   Salmonella  are major causative agents of infectious diseases in chicken. O 9  antigen as a part of lipopolysaccharide (LPS) is a predominant detected target for  Salmonella  infection. To identify the infection, an anti-O 9  monoclonal antibody (McAb)-based direct competitive enzyme-linked assay (O 9  Dc-ELISA) was developed after constraints were optimized; the establishment and application of O 9  Dc-ELISA, compared to two commercial kits and plate agglutination test (PAT), showed that O 9  Dc-ELISA could screen out more positive samples than the PAT method could and produce the same agreement rates with commercial kits in terms of sensitivity in addition to strong specificity to clinical serum samples.

A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken