English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Porcine diarrhea disease in newborn and suckling piglets due to infection with porcine epidemic diarrhea virus (PEDV) is a leading cause of economic loss in the pig industry globally. In this study, we investigated the molecular mechanism of the host innate immune response to PEDV infection. The expression dynamics of antiviral genes (e.g.,  RIG-1, PKR, OAS1, Mx1 , and  Mx2 ) and inflammatory cytokines (e.g.,  IFN- α , IFN- β,  TNF- α,  IL-6, IL-8 , and  IL-12 ) in porcine small intestinal epithelial (IPEC-J2) cells were analyzed following PEDV stimulation. The results showed that the expression of antiviral genes (e.g.,  PKR, OAS1 , and  Mx2 ) and inflammatory cytokines (e.g.,  IFN- α and  TNF- α) were significantly reduced within 0–4 h post-infection ( P  < 0.05). However, all antiviral genes and inflammatory cytokines were up-regulated from 12 to 24 h ( P  < 0.05), and cytopathic changes were observed during this time. The expression of  RIG-1, PKR, OAS1, Mx1 , and  Mx2  were significantly and positively correlated to each other during the entire infection ( P  < 0.01). The results suggested that the  RIG-1, PKR, OAS1, Mx1 , and  Mx2  genes may play an important role in PEDV infection in piglets. Initially, PEDV displayed cellular invasion by inhibiting  IFN- α transcription and interfering with the antiviral function of  PKR, OAS1 , and  Mx2 , ultimately induced an intense inflammatory response. The relationship between antiviral genes and inflammatory cytokines with PEDV infection at the cellular level provides a reference for studying the mechanism of resistance to PEDV infection in piglets.

Expression Pattern Analysis of Antiviral Genes and Inflammatory Cytokines in PEDV-Infected Porcine Intestinal Epithelial Cells