English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Objectives  This study aimed to identify and characterize extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae among clinical samples of companion animals.    Methods  A total of 346 non-duplicate Enterobacteriaceae isolates were collected between 2012 and 2016 from diseased cats ( n  = 115) and dogs ( n  = 231). The presence of  bla  ESBL , PMQR genes, and the azithromycin resistance gene  mph(A)  was confirmed by PCR and sequencing of  bla  genes. Isolates were further characterized by antimicrobial resistance profiling, multilocus sequence typing, phylogenetic grouping, identification of mutations in the QRDR of  gyrA  and  parC , and screening for virulence-associated genes.    Results  Among the 346 isolates, 72 (20.8%) were confirmed ESBL producers [58  Escherichia coli (E. coli) , 11  Klebsiella pneumoniae (K. pneumoniae) , and 3  Enterobacter cloacae ]. The strains were cultured from urine ( n  = 45), skin and skin wounds ( n  = 8), abscesses ( n  = 6), surgical sites ( n  = 6), bile ( n  = 4), and other sites ( n  = 3). ESBL genes included  bla  CTX-M-1 ,  14 ,  15 ,  27 ,  55 , and  bla  SHV-12 , predominantly  bla  CTX-M-15  (54.8%, 40/73), and  bla  CTX-M-1  (24.7%, 18/73). Further genes included  qnrB  (4.2%, 3/72),  qnrS  (9.7%, 7/72),  aac(6 ’ )-Ib-cr  (47.2%, 34/72), and  mph(A)  (38.9%, 28/72). Seventeen (23.6%) isolates belonged to the major lineages of human pathogenic  K. pneumoniae  ST11, ST15, and ST147 and  E. coli  ST131. The most prevalent ST was  E. coli  ST410 belonging to phylogenetic group C.    Conclusion  The high prevalence of ESBL producing clinical Enterobacteriaceae from cats and dogs in Switzerland and the presence of highly virulent human-related  K. pneumoniae  and  E. coli  clones raises concern about transmission prevention as well as infection management and prevention in veterinary medicine.

frontiers in veterinary science

High Prevalence of Extended-Spectrum β-Lactamase Producing Enterobacteriaceae Among Clinical Isolates From Cats and Dogs Admitted to a Veterinary Hospital in Switzerland