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Although etiolated  Arabidopsis thaliana  seedlings are widely used as a model to study the de-etiolation process, the etiolation itself at the molecular level still needs elucidation. Here, we monitored the etiolation dynamics for wild type  A. thaliana  seedlings and lutein-deficient ( lut2 ) mutant between 2 and 12 days of their growth in the absence of light. We analyzed the shape of the apex, the growth rate, the carotenoids and protochlorophyllide (Pchlide) accumulation, and the light-dependent protochlorophyllide oxidoreductase ( LPOR ) transcripts. Differences concerning the apical hook curvature and cotyledon opening among seedlings of the same age were observed, mostly after day 6 of the culture. We categorized the observed apex shapes and presented quantitatively how distribution among the categories changed during 12 days of seedling growth. The Pchlide 654 /Pchlide 633  ratio, corresponding to the amount of the photoactive Pchlide, was the highest in the youngest seedlings, and decreased with their age.  LPORA ,  LPORB , and  LPORC  transcripts were detected in etiolated seedlings, and their content decreased during seedling growth. Expression of  SAG12  or  SAG13  senescence markers, depletion in antioxidants, and excess ion leakage were not observed during the etiolation. Lack of lutein in the  lut2  mutant resulted in slow Pchlide accumulation and affected other xanthophyll composition.

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Dynamics of Etiolation Monitored by Seedling Morphology, Carotenoid Composition, Antioxidant Level, and Photoactivity of Protochlorophyllide in Arabidopsis thaliana