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WRKYs play important roles in plant metabolism, but their regulation mechanism in  Rehmannia glutinosa  remains elusive. In this study, 37 putative WRKY transcription factors (TFs) with complete WRKY domain from  R. glutinosa  transcriptome sequence data were identified. Based on their conserved domains and zinc finger motif, the  R. glutinosa  WRKY TFs were divided into five groups. Structural feature analysis shows that the 37 RgWRKY proteins contain WRKYGQK/GKK domains and a C2H2/C2HC-type zinc finger structure. To identify the function of  RgWRKY  members involved in acteoside biosynthesis, transcriptional profiles of 37  RgWRKYs  in hairy roots under salicylic acid (SA), methyl jasmonate (MeJA), and hydrogen peroxide (H 2 O 2 ) treatments were systematically established using RNA-seq analysis. Based on the correlationship between the expression levels of  RgWRKY  genes and acteoside content,  RgWRKY7 ,  RgWRKY23 ,  RgWRKY34 ,  RgWRKY35 , and  RgWRKY37  were suggested to be involved in acteoside biosynthesis in  R. glutinosa , and  RgWRKY37  was selected for gene functional research. Overexpression of  RgWRKY37  increased the content of acteoside and total phenylethanoid glycosides (PhGs) in hairy roots and enhanced the transcript abundance of seven enzyme genes involved in the acteoside biosynthesis pathway. These results strongly suggest the involvement of the WRKY transcription factor in the regulation of acteoside biosynthesis.

frontiers in plant science

Transcriptome-Wide Identification of WRKY Transcription Factor and Functional Characterization of RgWRKY37 Involved in Acteoside Biosynthesis in Rehmannia glutinosa