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Flavonol synthase (FLS) is a key enzyme for the formation of flavonols, which are a subclass of the flavonoids. FLS catalyzes the conversion of dihydroflavonols to flavonols. The enzyme belongs to the 2-oxoglutarate-dependent dioxygenases (2-ODD) superfamily. We characterized the  FLS  gene family of  Brassica napus  that covers 13 genes, based on the genome sequence of the  B. napus  cultivar Express 617. The goal was to unravel which  BnaFLS  genes are relevant for seed flavonol accumulation in the amphidiploid species  B. napus . Two  BnaFLS1  homeologs were identified and shown to encode bifunctional enzymes. Both exhibit FLS activity as well as flavanone 3-hydroxylase (F3H) activity, which was demonstrated  in vivo  and  in planta .  BnaFLS1-1  and  -2  are capable of converting flavanones into dihydroflavonols and further into flavonols. Analysis of spatio-temporal transcription patterns revealed similar expression profiles of  BnaFLS1  genes. Both are mainly expressed in reproductive organs and co-expressed with the genes encoding early steps of flavonoid biosynthesis. Our results provide novel insights into flavonol biosynthesis in  B. napus  and contribute information for breeding targets with the aim to modify the flavonol content in rapeseed.

Characterization of the Brassica napus Flavonol Synthase Gene Family Reveals Bifunctional Flavonol Synthases