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Mitogen-activated protein kinase (MAPK) signaling is required for plant cell death responses to invading microbial pathogens. Iron- and reactive oxygen species (ROS)-dependent ferroptotic cell death occurs in rice ( Oryza sativa ) during an incompatible rice– Magnaporthe oryzae  interaction. Here, we show that rice MAP kinase (OsMEK2 and OsMPK1) signaling cascades are involved in iron- and ROS-dependent ferroptotic cell death responses of rice to  M. oryzae  infection using  OsMEK2  knock-out mutant and  OsMEK2  and  OsMPK1  overexpression rice plants. The OsMPK1:GFP and OsWRKY90:GFP transcription factor were localized to the nuclei, suggesting that OsMPK1 in the cytoplasm moves into the nuclei to interact with the WRKY90.  M. oryzae  infection in Δ Osmek2  knock-out plants did not trigger iron and ROS accumulation and lipid peroxidation, and also downregulated  OsMPK1, OsWRKY90, OsRbohB , and  OsPR-1b  expression. However, 35S: OsMEK2  overexpression induced ROS- and iron-dependent cell death in rice. The downstream MAP kinase ( OsMPK1 ) overexpression induced ROS- and iron-dependent ferroptotic cell death response to virulent  M. oryzae  infection. The small-molecule ferroptosis inhibitor ferrostatin-1 suppressed iron- and ROS-dependent ferroptotic cell death in 35S: OsMPK1  overexpression plants. However, the small-molecule inducer erastin triggered iron- and lipid ROS-dependent, but  OsMEK2 -independent, ferroptotic cell death during  M. oryzae  infection. Disease (susceptibility)-related cell death was lipid ROS-dependent, but iron-independent in the Δ Osmek2  knock-out mutant during the late  M. oryzae  infection stage. These combined results suggest that  OsMEK2  and  OsMPK1  expression positively regulates iron- and ROS-dependent ferroptotic cell death, and blast disease (susceptibility)-related cell death was ROS-dependent but iron-independent in rice– M. oryzae  interactions.

Mitogen-Activated Protein Kinase OsMEK2 and OsMPK1 Signaling Is Required for Ferroptotic Cell Death in Rice–Magnaporthe oryzae Interactions