English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Goji berry ( Lycium barbarum  L.) exposure to elevated CO 2  (eCO 2 ) for long periods reduces their sugar and secondary metabolite contents. However, sugar accumulation in fruit depends on photosynthesis and photoassimilate partitioning. This study aimed to explore photosynthesis, sugar content, and sucrose metabolism-related enzyme activities in goji berry leaves and fruits under ambient and eCO 2  levels, and identify the genes encoding  L. barbarum  acid invertase ( LBAI ),  L. barbarum  sucrose synthase ( LBSS ),  L. barbarum  sucrose phosphate synthase ( LBSPS ), and  L. barbarum  neutral invertase ( LBNI ), based on transcriptome profiling. Further, the characterization of four identified genes was analyzed including subcellular localization and expression patterns. In plants grown under eCO 2  for 90 or 120 days, the expression of the above-mentioned genes changed significantly as the photosynthetic rate increased. In addition, leaf and fruit sugar contents decreased, and the activities of four sucrose metabolism-related enzymes increased in leaves, while acid and neutral invertase increased in fruits. Protein sequence analysis demonstrated that  LBAI  and  LBNI  contain a conservative structure domain belonging to the glycosyl hydrolases (Glyco_hydro) family, and both  LBSS  and  LBSPS  belonging to the sucrose synthase (Sucrose_synth) and glycosyltransferase (Glycos_transf) family. Subcellular localization analysis showed that  LBAI ,  LBNI , and  LBSS  were all located in the nucleus, plasma membrane, and cytoplasm, while  LBSPS  was located in the plasma membrane. The expressions of  LBAI ,  LBSPS , and  LBNI  were high in the stems, whereas  LBSS  was predominantly expressed in the fruits. Our findings provide fundamental data on photosynthesis and sugar accumulation trends in goji berries under eCO 2  exposure.

frontiers in plant science

Effects of Elevated CO2 on Photosynthetic Accumulation, Sucrose Metabolism-Related Enzymes, and Genes Identification in Goji Berry (Lycium barbarum L.)