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The specified floral meristem will develop a pre-established number of floral organs and, thus, terminate the floral meristematic cells. The floral meristematic pool of cells is controlled, among some others, by WUSCHEL (WUS) and AGAMOUS (AG) transcription factors (TFs). Here, we demonstrate that the  SCI1  (  S tigma/style  c ell-cycle  i nhibitor 1 ) gene, a cell proliferation regulator, starts to be expressed since the floral meristem specification of  Nicotiana tabacum  and is expressed in all floral meristematic cells. Its expression is higher in the floral meristem and the organs being specified, and then it decreases from outside to inside whorls when the organs are differentiating.  SCI1  is co-expressed with  N. tabacum WUSCHEL  ( NtWUS ) in the floral meristem and the whorl primordia at very early developmental stages. Later in development,  SCI1  is co-expressed with  NAG1  ( N. tabacum AG ) in the floral meristem and specialized tissues of the pistil.  In silico  analyses identified  cis -regulatory elements for these TFs in the  SCI1  genomic sequence. Yeast one-hybrid and electrophoresis mobility shift assay demonstrated that both TFs interact with the  SCI1  promoter sequence. Additionally, the luciferase activity assay showed that NAG1 clearly activates  SCI1  expression, while NtWUS could not do so. Taken together, our results suggest that during floral development, the spatiotemporal regulation of  SCI1  by NtWUS and NAG1 may result in the maintenance or termination of proliferative cells in the floral meristem, respectively.

SCI1 Is a Direct Target of AGAMOUS and WUSCHEL and Is Specifically Expressed in the Floral Meristematic Cells