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NAC transcription factors are one of the largest transcription factor families having functions in a variety of stress responses. Few NACs have been reported for interactions between wheat and the wheat rust fungus  Puccinia triticina  ( Pt ). In this study, based on analysis of RNA-seq data from wheat line TcLr19 inoculated by  Pt , the NAC transcription factor  TaNAC069  was cloned from wheat, and its transcriptional activity and homologous dimer formation were verified. Quantitative real-time PCR analysis showed that the expression of  TaNAC069  was induced by  Pt  and associated signaling molecules. To further characterize the function of the  TaNAC069  gene in wheat resistance to  Pt , virus-induced gene silencing (VIGS) was utilized, and it revealed that  Pt  resistance in  TaNAC069 -silenced plants was significantly reduced. Potential interaction targets of TaNAC069 from wheat and  Pt  were screened and identified by yeast two-hybrid technology. Eukaryotic elongation factor  eEF1A , CBSX3 protein, and cold acclimation protein WCOR410c were screened by yeast one-hybrid technology. The results indicate that the  TaNAC069  gene plays a positive regulatory role in wheat resistance to  Pt , laying a good foundation to analyze the molecular mechanisms of  TaNAC069  and its functional role in wheat resistance to  Pt .

Functional Analysis of Wheat NAC Transcription Factor, TaNAC069, in Regulating Resistance of Wheat to Leaf Rust Fungus