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Lateral root development is known to be regulated by Aux/IAA-ARF modules in  Arabidopsis   thaliana . As components, several Aux/IAAs have participated in these Aux/IAA-ARF modules. In this study, to identify the biological function of IAA15 in plant developments, transgenic plant overexpressing the gain-of-function mutant of IAA15 (IAA15 P75S  OX) under the control of dexamethasone (DEX) inducible promoter, in which IAA15 protein was mutated by changing Pro-75 residue to Ser at the degron motif in conserved domain II, was constructed. As a result, we found that IAA15 P75S  OX plants show a decreased number of lateral roots. Coincidently,  IAA15  promoter-GUS reporter analysis revealed that  IAA15  transcripts were highly detected in all stages of developing lateral root tissues. It was also verified that the IAA15 P75S  protein is strongly stabilized against proteasome-mediated protein degradation by inhibiting its poly-ubiquitination, resulting in the transcriptional repression of auxin-responsive genes. In particular, transcript levels of  LBD16  and  LBD29 , which are positive regulators of lateral root formation, dramatically repressed in IAA15 P75S  OX plants. Furthermore, it was elucidated that IAA15 interacts with ARF7 and ARF19 and binds to the promoters of  LBD16  and  LBD29 , strongly suggesting that IAA15 represses lateral root formation through the transcriptional suppression of  LBD16  and  LBD29  by inhibiting ARF7 and ARF19 activity. Taken together, this study suggests that IAA15 also plays a key negative role in lateral root formation as a component of Aux/IAA-ARF modules.

A Gain-of-Function Mutant of IAA15 Inhibits Lateral Root Development by Transcriptional Repression of LBD Genes in Arabidopsis