English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Setaria viridis  (green foxtail) has been identified as a potential experimental model system to genetically and molecularly characterise the C 4  monocotyledonous grasses due to its small physical size, short generation time and prolific seed production, together with a sequenced and annotated genome.  Setaria viridis  is the wild ancestor of the cropping species, foxtail millet ( Setaria italica ), with both  Setaria  species sharing a close evolutionary relationship with the agronomically important species, maize, sorghum, and sugarcane, as well as the bioenergy feedstocks, switchgrass, and  Miscanthus . However, an efficient and reproducible transformation protocol is required to further advance the use of  S. viridis  to study the molecular genetics of C 4  monocotyledonous grasses. An efficient and reproducible protocol was established for  Agrobacterium tumefaciens -mediated transformation of  S. viridis  (Accession A10) regenerable callus material derived from mature seeds, a protocol that returned an average transformation efficiency of 6.3%. The efficiency of this protocol was the result of the: (i) use of mature embryo derived callus material; (ii) age of the seed used to induce callus formation; (iii) composition of the callus induction media, including the addition of the ethylene inhibitor, silver nitrate; (iv) use of a co-cultivation approach, and; (v) concentration of the selective agent. Our protocol furthers the use of  S. viridis  as an experimental model system to study the molecular genetics of C 4  monocotyledonous grasses for the potential future development of improved C 4  cropping species.

Robust and Reproducible Agrobacterium-Mediated Transformation System of the C4 Genetic Model Species Setaria viridis