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Powdery mildew (PM), caused by  Podosphaera xanthii , is a major threat to the global cucurbit yield. The molecular mechanisms underlying the PM resistance of pumpkin ( Cucurbita moschata  Duch.) are largely unknown. A homolog of the basic helix-loop-helix (bHLH) transcription factor was previously identified through a transcriptomic analysis of a PM-resistant pumpkin. In this study, this bHLH homolog in pumpkin has been functionally characterized. CmbHLH87 is present in the nucleus.  CmbHLH87  expression in the PM-resistant material was considerably downregulated by PM; and abscisic acid, methyl jasmonate, ethephon, and NaCl treatments induced  CmbHLH87  expression. Ectopic expression of  CmbHLH87  in tobacco plants alleviated the PM symptoms on the leaves, accelerated cell necrosis, and enhanced H 2 O 2  accumulation. The expression levels of  PR1a ,  PR5 , and  NPR1  were higher in the PM-infected transgenic plants than in PM-infected wild-type plants. Additionally, the chlorosis and yellowing of plant materials were less extensive and the concentration of bacteria at infection sites was lower in the transgenic tobacco plants than in the wild-type plants in response to bacterial wilt and scab pathogens.  CmbHLH87  may be useful for genetic engineering of novel pumpkin cultivars in the future.

Expression of Pumpkin CmbHLH87 Gene Improves Powdery Mildew Resistance in Tobacco