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Wound attack stimulates accumulation of abscisic acid (ABA) that activates a number of genes associated with wound suberization of plants. Cytochrome P450 fatty acid ω-hydroxylase CYP86A1 catalyzes ω-hydroxylation of fatty acids to form the ω-functionalized monomers that play a pivotal role in suberin synthesis. However, the transcriptional regulation of ABA signaling on  AchnCYP86A1  has not been characterized in kiwifruit. In this study,  AchnCYP86A1 , a kiwifruit homolog of  Arabidopsis AtCYP86A1 , was isolated.  AchnCYP86A1 -overexpressed  N. benthamiana  leaves displayed that the AchnCYP86A1 functioned as a fatty acid ω-hydroxylase associated with synthesis of suberin monomer. The regulatory function of three transcription factors (TFs, including AchnMYC2, AchnMYB41 and AchnMYB107) on  AchnCYP86A1  was identified. All the three TFs were localized in nucleus and could individually interact with  AchnCYP86A1  promoter to activate gene expression in yeast one-hybrid and dual-luciferase assays. The findings were further demonstrated in transient overexpressed  N. benthamiana , in which all TFs notably elevated the expression of aliphatic synthesis genes including  CYP86A1  and the accumulation of ω-hydroxyacids, α, ω-diacids, fatty acids and primary alcohols. Moreover, exogenous ABA induced the expression of  AchnMYC2 ,  AchnMYB41  and  AchnMYB107  that promoted  AchnCYP86A1  involving in suberin monomer formation. Contrary to the inductive effects of ABA, however, fluridone (an inhibitor of ABA biosynthesis) inhibited the three TFs expression and suberin monomer formation. These results indicate that AchnMYC2, AchnMYB41 and AchnMYB107 positively regulate suberin monomer synthesis by activating  AchnCYP86A1  promoter in response to ABA.

Three Transcription Activators of ABA Signaling Positively Regulate Suberin Monomer Synthesis by Activating Cytochrome P450 CYP86A1 in Kiwifruit