English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The phytopathogenic fungus  Leptosphaeria maculans  causes the blackleg disease on  Brassica napus , resulting in severe loss of rapeseed production. Breeding of resistant cultivars containing race-specific resistance genes is provably effective to combat this disease. While two allelic resistance genes  LepR3  and  Rlm2  recognizing  L. maculans  avirulence genes  AvrLm1  and  AvrLm2  at plant apoplastic space have been cloned in  B. napus , the downstream gene expression network underlying the resistance remains elusive. In this study, transgenic lines expressing  LepR3  and  Rlm2  were created in the susceptible “Westar” cultivar and inoculated with  L. maculans  isolates containing different sets of  AvrLm1  and  AvrLm2  for comparative transcriptomic analysis. Through grouping the RNA-seq data based on different levels of defense response, we find  LepR3  and  Rlm2  orchestrate a hierarchically regulated gene expression network, consisting of induced ABA acting independently of the disease reaction, activation of signal transduction pathways with gradually increasing intensity from compatible to incompatible interaction, and specifically induced enzymatic and chemical actions contributing to hypersensitive response with recognition of  AvrLm1  and  AvrLm2 . This study provides an unconventional investigation into  LepR3  and  Rlm2 -mediated plant defense machinery and adds novel insight into the interaction between surface-localized receptor-like proteins (RLPs) and apoplastic fungal pathogens.

Transcriptional Insight Into Brassica napus Resistance Genes LepR3 and Rlm2-Mediated Defense Response Against the Leptosphaeria maculans Infection