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Black shank caused by  Phytophthora nicotianae  is one of the most devastating diseases in tobacco production. In this study, we characterized a novel cytochromic resistance gene,  SoCYP85A1 , from spinach, which was upregulated in response to  P. nicotianae  infection. Overexpression of  SoCYP85A1  in tobacco resulted in remarkable resistance to pathogen inoculation, with diverse resistance levels in different transgenic lines. Meanwhile, a significant accumulation of castasterone (CS) was detected in transgenic plants when challenged with the pathogen. Moreover, activities of antioxidant enzymes were enhanced by  SoCYP85A1  in the transgenic lines as compared to those in the wild types inoculated with  P. nicotianae . In addition, the alteration of CS content resulted in interference of phytohormone homeostasis. Overall, these results demonstrate that  SoCYP85A1  can participate in the defense response to  P. nicotianae  through the involvement of defense enzymes and by interaction with certain phytohormones. Our findings suggest that  SoCYP85A1  could be used as a potential candidate gene for improving resistance to black shank disease in tobacco and other economic crops.

frontiers in plant science

Overexpression of SoCYP85A1 Increases the Accumulation of Castasterone and Confers Enhanced Black Shank Tolerance in Tobacco Through Modulation of the Antioxidant Enzymes’ Activities